Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection
KM Peck, AS Cockrell, BL Yount, T Scobey… - Journal of …, 2015 - Am Soc Microbiol
KM Peck, AS Cockrell, BL Yount, T Scobey, RS Baric, MT Heise
Journal of virology, 2015•Am Soc MicrobiolMiddle East respiratory syndrome coronavirus (MERS-CoV) utilizes dipeptidyl peptidase 4
(DPP4) as an entry receptor. Mouse DPP4 (mDPP4) does not support MERS-CoV entry;
however, changes at positions 288 and 330 can confer permissivity. Position 330 changes
the charge and glycosylation state of mDPP4. We show that glycosylation is a major factor
impacting DPP4 receptor function. These results provide insight into DPP4 species-specific
differences impacting MERS-CoV host range and may inform MERS-CoV mouse model …
(DPP4) as an entry receptor. Mouse DPP4 (mDPP4) does not support MERS-CoV entry;
however, changes at positions 288 and 330 can confer permissivity. Position 330 changes
the charge and glycosylation state of mDPP4. We show that glycosylation is a major factor
impacting DPP4 receptor function. These results provide insight into DPP4 species-specific
differences impacting MERS-CoV host range and may inform MERS-CoV mouse model …
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) utilizes dipeptidyl peptidase 4 (DPP4) as an entry receptor. Mouse DPP4 (mDPP4) does not support MERS-CoV entry; however, changes at positions 288 and 330 can confer permissivity. Position 330 changes the charge and glycosylation state of mDPP4. We show that glycosylation is a major factor impacting DPP4 receptor function. These results provide insight into DPP4 species-specific differences impacting MERS-CoV host range and may inform MERS-CoV mouse model development.
American Society for Microbiology