[HTML][HTML] Generation of a stable activated thrombin activable fibrinolysis inhibitor variant
E Ceresa, K Van de Borne, M Peeters… - Journal of Biological …, 2006 - ASBMB
Activated thrombin activable fibrinolysis inhibitor (TAFIa), generated upon activation of TAFI,
exerts an antifibrinolytic effect. TAFIa is a thermolabile enzyme, inactivated through a
conformational change. The objective of the current study was to generate a stable variant of
human TAFIa. Using a site-directed as well as a random mutagenesis approach to generate
a library of TAFI mutants, we identified two mutations that increase TAFIa stability, ie a Ser
305 to Cys and a Thr 329 to Ile mutation, respectively. Combining these mutations in TAFI …
exerts an antifibrinolytic effect. TAFIa is a thermolabile enzyme, inactivated through a
conformational change. The objective of the current study was to generate a stable variant of
human TAFIa. Using a site-directed as well as a random mutagenesis approach to generate
a library of TAFI mutants, we identified two mutations that increase TAFIa stability, ie a Ser
305 to Cys and a Thr 329 to Ile mutation, respectively. Combining these mutations in TAFI …