Interleukin-3 and encephalitogenic activity of SJL/J myelin basic protein-specific T cell lines
ML Zhao, JQ Xia, RB Fritz - Journal of neuroimmunology, 1993 - Elsevier
Myelin basic protein (MBP)-specific SJL/JT cells were cultured in normal growth medium or
growth medium supplemented with 10% culture supernatant from WEHI-3 cells, a source of
interleukin-3 (IL-3), or with recombinant IL-3. T cell lines cultured with IL-3 supplementation
were more encephalitogenic compared to parallel lines cultured without this supplement.
There was little difference in antigen-specific proliferative response or expression of cell
surface markers CD3, CD4, CD8, IL-2R, or α/βTCR in the parallel lines. Supernatant fluids …
growth medium supplemented with 10% culture supernatant from WEHI-3 cells, a source of
interleukin-3 (IL-3), or with recombinant IL-3. T cell lines cultured with IL-3 supplementation
were more encephalitogenic compared to parallel lines cultured without this supplement.
There was little difference in antigen-specific proliferative response or expression of cell
surface markers CD3, CD4, CD8, IL-2R, or α/βTCR in the parallel lines. Supernatant fluids …