Production of interleukin-3 by murine central nervous system neurons
Y Konishi, M Kamegai, K Takahashi, T Kunishita… - Neuroscience …, 1994 - Elsevier
Y Konishi, M Kamegai, K Takahashi, T Kunishita, T Tabira
Neuroscience letters, 1994•ElsevierWe examined expression and production of interleukin-3 (IL-3) mRNA and IL-3 protein in
mouse primary cultured neurons and glia by the reverse transcription and polymerase chain
reaction and a bioassay using an IL-3-dependent cell line. IL-3 mRNA was demonstrated
mainly in hippocampal neurons but not in glia, while a small but definite production of
bioactive IL-3 was detected in septal and hippocampal neuronal cultures. Thus,
endogenous IL-3 might be produced by certain neurons in situ.
mouse primary cultured neurons and glia by the reverse transcription and polymerase chain
reaction and a bioassay using an IL-3-dependent cell line. IL-3 mRNA was demonstrated
mainly in hippocampal neurons but not in glia, while a small but definite production of
bioactive IL-3 was detected in septal and hippocampal neuronal cultures. Thus,
endogenous IL-3 might be produced by certain neurons in situ.
Abstract
We examined expression and production of interleukin-3 (IL-3) mRNA and IL-3 protein in mouse primary cultured neurons and glia by the reverse transcription and polymerase chain reaction and a bioassay using an IL-3-dependent cell line. IL-3 mRNA was demonstrated mainly in hippocampal neurons but not in glia, while a small but definite production of bioactive IL-3 was detected in septal and hippocampal neuronal cultures. Thus, endogenous IL-3 might be produced by certain neurons in situ.
Elsevier