[HTML][HTML] HTLV-1 infects human mesenchymal stromal cell in vitro and modifies their phenotypic characteristics
ES Rodrigues, MD de Macedo, MT Pinto, MD Orellana… - Virology, 2014 - Elsevier
ES Rodrigues, MD de Macedo, MT Pinto, MD Orellana, MCR Junior, DAR de Magalhães…
Virology, 2014•ElsevierThe typical characteristics of mesenchymal stem cells (MSCs) can be affected by
inflammatory microenvironment; however, the exact contribution of HTLV-1 to MSC
dysfunction remains to be elucidated. In this study, we demonstrated that MSC cell surface
molecules VCAM-1 and ICAM-1 are upregulated by contact with HTLV-1, and HLA-DR was
most highly expressed in MSCs co-cultured with MT2 cells. The expression levels of VCAM-
1 and HLA-DR were increased in MSCs cultured in the presence of PBMCs isolated from …
inflammatory microenvironment; however, the exact contribution of HTLV-1 to MSC
dysfunction remains to be elucidated. In this study, we demonstrated that MSC cell surface
molecules VCAM-1 and ICAM-1 are upregulated by contact with HTLV-1, and HLA-DR was
most highly expressed in MSCs co-cultured with MT2 cells. The expression levels of VCAM-
1 and HLA-DR were increased in MSCs cultured in the presence of PBMCs isolated from …
Abstract
The typical characteristics of mesenchymal stem cells (MSCs) can be affected by inflammatory microenvironment; however, the exact contribution of HTLV-1 to MSC dysfunction remains to be elucidated. In this study, we demonstrated that MSC cell surface molecules VCAM-1 and ICAM-1 are upregulated by contact with HTLV-1, and HLA-DR was most highly expressed in MSCs co-cultured with MT2 cells. The expression levels of VCAM-1 and HLA-DR were increased in MSCs cultured in the presence of PBMCs isolated from HTLV-1-infected symptomatic individuals compared with those cultured with cells from asymptomatic infected individuals or healthy subjects. HTLV-1 does not impair the MSC differentiation process into osteocytes and adipocytes. In addition, MSCs were efficiently infected with HTLV-1 in vitro through direct contact with HTLV-1-infected cells; however, cell-free virus particles were not capable of causing infection. In summary, HTLV-1 can alter MSC function, and this mechanism may contribute to the pathogenesis of this viral infection.
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