[HTML][HTML] Induction of endogenous uncoupling protein 3 suppresses mitochondrial oxidant emission during fatty acid-supported respiration

EJ Anderson, H Yamazaki, PD Neufer - Journal of Biological Chemistry, 2007 - ASBMB
Uncoupling protein 3 (UCP3) expression increases dramatically in skeletal muscle under
metabolic states associated with elevated lipid metabolism, yet the function of UCP3 in a
physiological context remains controversial. Here, in situ mitochondrial H 2 O 2 emission
and respiration were measured in permeabilized fiber bundles prepared from both rat and
mouse (wild-type) gastrocnemius muscle after a single bout of exercise plus 18 h of recovery
(Ex/R) that induced a∼ 2–4-fold increase in UCP3 protein. Elevated uncoupling activity (ie …