The use of anti-CD3 and anti-CD28 monoclonal antibodies to clone and expand human antigen-specific T cells
SR Riddell, PD Greenberg - Journal of immunological methods, 1990 - Elsevier
Antigen-specific T cell clones are useful reagents for studies of the fine specificity of antigen
recognition and of potential therapeutic use in adoptive immunotherapy for human viral and
malignant diseases. Culture methods which require antigen and APC for stimulation can be
problematic for the generation and long-term growth of human virus and tumor-specific T
cells. We have developed an alternative culture method using monoclonal antibodies to T
cell activation molecules, CD3 and CD28, as stimulation to efficiently grow CD4+ and CD8+ …
recognition and of potential therapeutic use in adoptive immunotherapy for human viral and
malignant diseases. Culture methods which require antigen and APC for stimulation can be
problematic for the generation and long-term growth of human virus and tumor-specific T
cells. We have developed an alternative culture method using monoclonal antibodies to T
cell activation molecules, CD3 and CD28, as stimulation to efficiently grow CD4+ and CD8+ …