A combined 3D tissue engineered in vitro/in silico lung tumor model for predicting drug effectiveness in specific mutational backgrounds
C Göttlich, LC Müller, M Kunz, F Schmitt… - JoVE (Journal of …, 2016 - jove.com
In the present study, we combined an in vitro 3D lung tumor model with an in silico model to
optimize predictions of drug response based on a specific mutational background. The
model is generated on a decellularized porcine scaffold that reproduces tissue-specific
characteristics regarding extracellular matrix composition and architecture including the
basement membrane. We standardized a protocol that allows artificial tumor tissue
generation within 14 days including three days of drug treatment. Our article provides …
optimize predictions of drug response based on a specific mutational background. The
model is generated on a decellularized porcine scaffold that reproduces tissue-specific
characteristics regarding extracellular matrix composition and architecture including the
basement membrane. We standardized a protocol that allows artificial tumor tissue
generation within 14 days including three days of drug treatment. Our article provides …