qPrimerDepot: a primer database for quantitative real time PCR

W Cui, DD Taub, K Gardner - Nucleic acids research, 2007 - academic.oup.com
W Cui, DD Taub, K Gardner
Nucleic acids research, 2007academic.oup.com
Gene expression studies employing high throughput real time PCR methods require finding
uniform conditions for optimal amplification of multiple targets, often a daunting task. We
developed a primer database, qPrimerDepot, which provides optimized primers for all
human and mouse RefSeq genes. These primers are designed to amplify desired templates
under unified annealing temperature. For most intron-bearing genes, primers flank one of
the largest introns thus minimizing background noise due to genomic DNA contamination …
Abstract
Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at and .
Oxford University Press