[HTML][HTML] Green fluorescent protein expression in dendritic cells enhances their immunogenicity and elicits specific cytotoxic T-cell responses in humans
F Re, R Srinivasan, T Igarashi, F Marincola… - Experimental …, 2004 - Elsevier
OBJECTIVE: Green fluorescent protein (GFP) has been used to monitor and select cells
transduced with vectors encoding other transgenes of interest. We investigated the
immunogenic nature of GFP in humans and further explored whether this xenoprotein could
be used as a functional adjuvant to enhance T-cell immunity to the melanoma tumor antigen
MART1. METHODS: Peripheral blood lymphocytes from healthy donors were stimulated by
autologous dendritic cells expressing GFP, then cloned by limiting dilution and tested for …
transduced with vectors encoding other transgenes of interest. We investigated the
immunogenic nature of GFP in humans and further explored whether this xenoprotein could
be used as a functional adjuvant to enhance T-cell immunity to the melanoma tumor antigen
MART1. METHODS: Peripheral blood lymphocytes from healthy donors were stimulated by
autologous dendritic cells expressing GFP, then cloned by limiting dilution and tested for …