Cytotoxicity mediated by T cells and natural killer cells is greatly impaired in perforin-deficient mice

D Kägi, B Ledermann, K Bürki, P Seiler, B Odermatt… - Nature, 1994 - nature.com
D Kägi, B Ledermann, K Bürki, P Seiler, B Odermatt, KJ Olsen, ER Podack, RM Zinkernagel…
Nature, 1994nature.com
Perforin-deficient mice have been generated by homologous recombination to determine
whether the effects of CDS+ cytolytic T cells and natural killer cells are mediated by pore
formation involving perform. These mice are viable and fertile and have normal numbers of
CDS+ T cells and natural killer cells which do not lyse virus-infected or allogeneic fibroblasts
or natural killer target cells in vitro. The mice fail to clear lymphocytic choriomeningitis virus
and they eliminate fibrosarcoma tumour cells with reduced efficiency. Perforin is therefore a …
Abstract
Perforin-deficient mice have been generated by homologous recombination to determine whether the effects of CDS+ cytolytic T cells and natural killer cells are mediated by pore formation involving perform. These mice are viable and fertile and have normal numbers of CDS+ T cells and natural killer cells which do not lyse virus-infected or allogeneic fibroblasts or natural killer target cells in vitro. The mice fail to clear lymphocytic choriomeningitis virus and they eliminate fibrosarcoma tumour cells with reduced efficiency. Perforin is therefore a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.
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