[HTML][HTML] Regulation of an activated S6 kinase 1 variant reveals a novel mammalian target of rapamycin phosphorylation site
M Saitoh, N Pullen, P Brennan, D Cantrell… - Journal of Biological …, 2002 - ASBMB
A critical step in S6 kinase 1 (S6K1) activation is Thr 229 phosphorylation in the activation
loop by the phosphoinositide-dependent protein kinase (PDK1). Thr 229 phosphorylation
requires prior phosphorylation of the Ser/Thr-Pro sites in the autoinhibitory domain and Thr
389 in the linker domain, consistent with PDK1 more effectively catalyzing Thr 229
phosphorylation in a variant harboring acidic residues in these positions (S6K1-E389D 3 E).
S6K1-E389D 3 E has high basal activity and exhibits partial resistance to rapamycin and …
loop by the phosphoinositide-dependent protein kinase (PDK1). Thr 229 phosphorylation
requires prior phosphorylation of the Ser/Thr-Pro sites in the autoinhibitory domain and Thr
389 in the linker domain, consistent with PDK1 more effectively catalyzing Thr 229
phosphorylation in a variant harboring acidic residues in these positions (S6K1-E389D 3 E).
S6K1-E389D 3 E has high basal activity and exhibits partial resistance to rapamycin and …