Characterization of a conditional interleukin‐1 receptor 1 mouse mutant using the Cre/LoxP system

WH Abdulaal, CR Walker, R Costello… - European journal of …, 2016 - Wiley Online Library
WH Abdulaal, CR Walker, R Costello, E Redondo‐Castro, IA Mufazalov, A Papaemmanouil…
European journal of immunology, 2016Wiley Online Library
IL‐1 is a key cytokine known to drive chronic inflammation and to regulate many
physiological, immunological, and neuroimmunological responses via actions on diverse
cell types of the body. To determine the mechanisms of IL‐1 actions as part of the
inflammatory response in vivo, we generated a conditional IL‐1 receptor 1 (IL‐1R1) mouse
mutant using the Cre/LoxP system (IL‐1R1fl/fl). In the mutant generated, exon 5, which
encodes part of the extracellular‐binding region of the receptor, is flanked by LoxP sites …
IL‐1 is a key cytokine known to drive chronic inflammation and to regulate many physiological, immunological, and neuroimmunological responses via actions on diverse cell types of the body. To determine the mechanisms of IL‐1 actions as part of the inflammatory response in vivo, we generated a conditional IL‐1 receptor 1 (IL‐1R1) mouse mutant using the Cre/LoxP system (IL‐1R1fl/fl). In the mutant generated, exon 5, which encodes part of the extracellular‐binding region of the receptor, is flanked by LoxP sites, thereby inactivating the two previously described functional IL‐1R1 gene transcripts after Cre‐mediated recombination. Using keratin 14‐Cre driver mice, new IL‐1R1 deficient (−/−) mice were subsequently generated, in which all signaling IL‐1 receptor isoforms are deleted ubiquitously. Furthermore, using vav‐iCre driver mice, we deleted IL‐1 receptor isoforms in the hematopoietic system. In these mice, we show that both the IL‐17 and IL‐22 cytokine response is reduced, when mice are challenged by the helminth Trichuris muris. We are currently crossing IL‐1R1fl/fl mice with different Cre‐expressing mice in order to study mechanisms of acute and chronic inflammatory diseases.
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