[HTML][HTML] Extra-long Gαs variant XLαs protein escapes activation-induced subcellular redistribution and is able to provide sustained signaling
Z Liu, S Turan, VL Wehbi, JP Vilardaga… - Journal of Biological …, 2011 - ASBMB
Murine models indicate that Gαs and its extra-long variant XLαs, both of which are derived
from GNAS, markedly differ regarding their cellular actions, but these differences are
unknown. Here we investigated activation-induced trafficking of Gαs and XLαs, using
immunofluorescence microscopy, cell fractionation, and total internal reflection fluorescence
microscopy. In transfected cells, XLαs remained localized to the plasma membrane,
whereas Gαs redistributed to the cytosol after activation by GTPase-inhibiting mutations …
from GNAS, markedly differ regarding their cellular actions, but these differences are
unknown. Here we investigated activation-induced trafficking of Gαs and XLαs, using
immunofluorescence microscopy, cell fractionation, and total internal reflection fluorescence
microscopy. In transfected cells, XLαs remained localized to the plasma membrane,
whereas Gαs redistributed to the cytosol after activation by GTPase-inhibiting mutations …