Extracellular vesicles (EVs) are newly discovered subcellular components that play
important roles in many biological signaling functions during physiological and pathological
states. The isolation of EVs continues to be a major challenge in this field, due to limitations
intrinsic to each technique. The differential ultracentrifugation with density gradient
centrifugation method is a commonly used approach and is considered to be the gold
standard procedure for EV isolation. However, this procedure is time-consuming, labor …