[HTML][HTML] MMP7 Shedding of Syndecan-1 Facilitates Re-Epithelialization by Affecting α2β1 Integrin Activation

P Chen, LE Abacherli, ST Nadler, Y Wang, Q Li… - PloS one, 2009 - journals.plos.org
P Chen, LE Abacherli, ST Nadler, Y Wang, Q Li, WC Parks
PloS one, 2009journals.plos.org
Background Lung injury promotes the expression of matrix metalloproteinase-7 (MMP7,
matrilysin), which is required for neutrophil recruitment and re-epithelialization. MMP7
governs the lung inflammatory response through the shedding of syndecan-1. Because
inflammation and repair are related events, we evaluated the role of syndecan-1 shedding in
lung re-epithelialization. Methodology/Principal Finding Epithelial injury induced syndecan-
1 shedding from wild-type epithelium but not from Mmp7−/− mice in vitro and in vivo …
Background
Lung injury promotes the expression of matrix metalloproteinase-7 (MMP7, matrilysin), which is required for neutrophil recruitment and re-epithelialization. MMP7 governs the lung inflammatory response through the shedding of syndecan-1. Because inflammation and repair are related events, we evaluated the role of syndecan-1 shedding in lung re-epithelialization.
Methodology/Principal Finding
Epithelial injury induced syndecan-1 shedding from wild-type epithelium but not from Mmp7−/− mice in vitro and in vivo. Moreover, cell migration and wound closure was enhanced by MMP7 shedding of syndecan-1. Additionally, we found that syndecan-1 augmented cell adhesion to collagen by controlling the affinity state of the α2β1 integrin.
Conclusion/Significance
MMP7 shedding of syndecan-1 facilitates wound closure by causing the α2β1 integrin to assume a less active conformation thereby removing restrictions to migration. MMP7 acts in the lungs to regulate inflammation and repair, and our data now show that both these functions are controlled through the shedding of syndecan-1.
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