[HTML][HTML] Stabilization of the MDM2 oncoprotein by mutant p53
Y Peng, L Chen, C Li, W Lu, S Agrawal… - Journal of Biological …, 2001 - ASBMB
MDM2 is a short-lived protein that regulates p53 degradation. We report here that transient
coexpression of MDM2 and several p53 hotspot mutants resulted in stabilization and
increased expression of MDM2. Ectopic expression of the mutant p53 175H allele by
recombinant adenovirus infection or stable transfection also stabilized endogenous MDM2
inp53-null cells. A panel of human tumor cell lines expressing different endogenous mutant
p53 alleles also contained stabilized nuclear MDM2 at elevated levels when compared …
coexpression of MDM2 and several p53 hotspot mutants resulted in stabilization and
increased expression of MDM2. Ectopic expression of the mutant p53 175H allele by
recombinant adenovirus infection or stable transfection also stabilized endogenous MDM2
inp53-null cells. A panel of human tumor cell lines expressing different endogenous mutant
p53 alleles also contained stabilized nuclear MDM2 at elevated levels when compared …