[HTML][HTML] A novel method for the assessment of cellular composition and beta-cell viability in human islet preparations
Current methodologies to evaluate islet cell viability are largely based on tests that assess
the exclusion of DNA-binding dyes. While these tests identify cells that have lost selective
membrane permeability, they do not allow us to recognize apoptotic cells, which do not yet
stain with DNA-binding dyes. Furthermore, current methods of analysis do not discriminate
between cell subsets in the preparation and, in particular, they do not allow for selectively
defining β-cell viability. For these reasons we have developed novel methods for the specific …
the exclusion of DNA-binding dyes. While these tests identify cells that have lost selective
membrane permeability, they do not allow us to recognize apoptotic cells, which do not yet
stain with DNA-binding dyes. Furthermore, current methods of analysis do not discriminate
between cell subsets in the preparation and, in particular, they do not allow for selectively
defining β-cell viability. For these reasons we have developed novel methods for the specific …