[HTML][HTML] Determination of the role of the human RNase H1 in the pharmacology of DNA-like antisense drugs
H Wu, WF Lima, H Zhang, A Fan, H Sun… - Journal of Biological …, 2004 - ASBMB
Although ribonuclease H activity has long been implicated as a molecular mechanism by
which DNA-like oligonucleotides induce degradation of target RNAs, definitive proof that
one or more RNase H is responsible is lacking. To date, two RNase H enzymes (H1 and H2)
have been cloned and shown to be expressed in human cells and tissues. To determine the
role of RNase H1 in the mechanism of action of DNA-like antisense drugs, we varied the
levels of the enzyme in human cells and mouse liver and determined the correlation of those …
which DNA-like oligonucleotides induce degradation of target RNAs, definitive proof that
one or more RNase H is responsible is lacking. To date, two RNase H enzymes (H1 and H2)
have been cloned and shown to be expressed in human cells and tissues. To determine the
role of RNase H1 in the mechanism of action of DNA-like antisense drugs, we varied the
levels of the enzyme in human cells and mouse liver and determined the correlation of those …