Saving cells from ultrasound-induced apoptosis: quantification of cell death and uptake following sonication and effects of targeted calcium chelation
JD Hutcheson, RK Schlicher, HK Hicks… - Ultrasound in medicine & …, 2010 - Elsevier
Applications of ultrasound for noninvasive drug and gene delivery have been limited by
associated cell death as a result of sonication. In this study, we sought to quantify the
distribution of cellular bioeffects caused by low-frequency ultrasound (24 kHz) and test the
hypothesis that Ca2+ chelation after sonication can shift this distribution by saving cells from
death by apoptosis. Using flow cytometry, we quantitatively categorized sonicated cells
among four populations:(i) cells that appear largely unaffected,(ii) cells reversibly …
associated cell death as a result of sonication. In this study, we sought to quantify the
distribution of cellular bioeffects caused by low-frequency ultrasound (24 kHz) and test the
hypothesis that Ca2+ chelation after sonication can shift this distribution by saving cells from
death by apoptosis. Using flow cytometry, we quantitatively categorized sonicated cells
among four populations:(i) cells that appear largely unaffected,(ii) cells reversibly …