[HTML][HTML] NADPH oxidase-derived reactive oxygen species increases expression of monocyte chemotactic factor genes in cultured adipocytes

CY Han, T Umemoto, M Omer, LJ Den Hartigh… - Journal of Biological …, 2012 - ASBMB
Excess glucose and free fatty acids delivered to adipose tissue causes local inflammation,
which contributes to insulin resistance. Glucose and palmitate generate reactive oxygen
species (ROS) in adipocytes, leading to monocyte chemotactic factor gene expression.
Docosahexaenoate (DHA) has the opposite effect. In this study, we evaluated the potential
sources of ROS in the presence of excess nutrients. Differentiated 3T3-L1 adipocytes were
exposed to palmitate and DHA (250 μm) in either 5 or 25 mm glucose to evaluate the …