Simplified human neutrophil extracellular traps (NETs) isolation and handling

S Najmeh, J Cools-Lartigue, B Giannias… - JoVE (Journal of …, 2015 - jove.com
Neutrophil Extracellular Traps (NETs) have been recently identified as part of the
neutrophil's antimicrobial armamentarium. Apart from their role in fighting infections, recent
research has demonstrated that they may be involved in many other disease processes,
including cancer progression. Isolating purified NETs is a crucial element to allow the study
of these functions. In this video, we demonstrate a simplified method of cell free NET
isolation from human whole blood using readily available reagents. Isolated NETs can then …