Photoconversion in orange and red fluorescent proteins
GJ Kremers, KL Hazelwood, CS Murphy… - Nature …, 2009 - nature.com
GJ Kremers, KL Hazelwood, CS Murphy, MW Davidson, DW Piston
Nature methods, 2009•nature.comWe found that photoconversion is fairly common among orange and red fluorescent
proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red
fluorescent proteins could be switched to a green state, and two orange variants could be
photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter
applications, and they exhibited the most red-shifted excitation of all fluorescent proteins
described to date.
proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red
fluorescent proteins could be switched to a green state, and two orange variants could be
photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter
applications, and they exhibited the most red-shifted excitation of all fluorescent proteins
described to date.
Abstract
We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.
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