Expression of human glucose transporters in Xenopus oocytes: kinetic characterization and substrate specificities of the erythrocyte, liver, and brain isoforms

GW Gould, HM Thomas, TJ Jess, GI Bell - Biochemistry, 1991 - ACS Publications
GW Gould, HM Thomas, TJ Jess, GI Bell
Biochemistry, 1991ACS Publications
Materials and Methods Materials. Collagenase was obtained from Worthington Scientific Ltd.
Sodium diguanosine triphosphate was pur-chased from Pharmacia. SP6 polymerase,
RNasin, and nu-cleotides were purchased from Promega Biotech. Penicillin/streptomycin
solutionand all restriction endonucleases were from Gibco/BRL (Paisley, Glasgow). 3-0-
Methyl-o-[l-
Materials and Methods
Materials. Collagenase was obtained from Worthington Scientific Ltd. Sodium diguanosine triphosphate was pur-chased from Pharmacia. SP6 polymerase, RNasin, and nu-cleotides were purchased from Promega Biotech. Penicillin/streptomycin solutionand all restriction endonucleases were from Gibco/BRL (Paisley, Glasgow). 3-0-Methyl-o-[l-
ACS Publications