Increased expression of ADAM 9 and ADAM 15 mRNA in pancreatic cancer

D Yamada, K Ohuchida, K Mizumoto… - Anticancer …, 2007 - ar.iiarjournals.org
D Yamada, K Ohuchida, K Mizumoto, S Ohhashi, J Yu, T Egami, H Fujita, E Nagai, M Tanaka
Anticancer research, 2007ar.iiarjournals.org
Background: A disintegrin and metalloproteases (ADAMs) comprise a multifunctional family
of membrane-anchored proteins. ADAM 9 and ADAM 15 are involved in cell migration and
invasion. Expression of ADAM 9 and ADAM 15 was reported to be altered in several types of
cancer. Materials and Methods: Quantitative real-time reverse transcription-polymerase
chain reaction was performed to measure the expression of ADAM 9 mRNA in bulk
pancreatic tissues. Results showed no significant difference in the expression of ADAM 9 …
Background
A disintegrin and metalloproteases (ADAMs) comprise a multifunctional family of membrane-anchored proteins. ADAM 9 and ADAM 15 are involved in cell migration and invasion. Expression of ADAM 9 and ADAM 15 was reported to be altered in several types of cancer.
Materials and Methods
Quantitative real-time reverse transcription-polymerase chain reaction was performed to measure the expression of ADAM 9 mRNA in bulk pancreatic tissues. Results showed no significant difference in the expression of ADAM 9 mRNA between pancreatic cancer and non-neoplastic pancreas. Primary cultured pancreatic fibroblasts also expressed ADAM 9 mRNA. Therefore, a laser microdissection and pressure catapulting technique was employed to isolate cancer cells from tumor tissues. The expression of ADAM 9 and ADAM 15 mRNA was measured in microdissected samples (cancer cells, n=11; normal epithelial cells, n=13 for ADAM 9; cancer cells, n=9; normal epithelial cells, n=9 for ADAM 15).
Results
Pancreatic cancer cells expressed significantly higher levels of ADAM 9 and ADAM 15 mRNA than did normal pancreatic epithelial cells (p=0.016 for ADAM 9; p=0.004 for ADAM 15).
Conclusion
ADAM 9 and ADAM 15 are involved in pancreatic cancer. Microdissection-based analysis appears to be indispensable for the accurate analysis of the expression of certain ADAM family members in pancreatic cancer.
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