Lipidomic “deep profiling”: an enhanced workflow to reveal new molecular species of signaling lipids
Analytical chemistry, 2014•ACS Publications
Current mass spectrometry-based lipidomics aims to comprehensively cover wide ranges of
lipid classes. We introduce a strategy to capture phospho-monoester lipids and improve the
detection of long-chain base phosphates (LCB-Ps, eg, sphingosine-1-phosphate). Ten novel
LCB-Ps (d18: 2, t20: 1, odd carbon forms) were discovered and characterized in tissues from
human and mouse, as well in D. melanogaster and S. cerevisiae. These findings have
immediate relevance for our understanding of sphingosine-1-phosphate biosynthesis …
lipid classes. We introduce a strategy to capture phospho-monoester lipids and improve the
detection of long-chain base phosphates (LCB-Ps, eg, sphingosine-1-phosphate). Ten novel
LCB-Ps (d18: 2, t20: 1, odd carbon forms) were discovered and characterized in tissues from
human and mouse, as well in D. melanogaster and S. cerevisiae. These findings have
immediate relevance for our understanding of sphingosine-1-phosphate biosynthesis …
Current mass spectrometry-based lipidomics aims to comprehensively cover wide ranges of lipid classes. We introduce a strategy to capture phospho-monoester lipids and improve the detection of long-chain base phosphates (LCB-Ps, e.g., sphingosine-1-phosphate). Ten novel LCB-Ps (d18:2, t20:1, odd carbon forms) were discovered and characterized in tissues from human and mouse, as well in D. melanogaster and S. cerevisiae. These findings have immediate relevance for our understanding of sphingosine-1-phosphate biosynthesis, signaling, and degradation.
ACS Publications