[HTML][HTML] Tracing the fates of site-specifically introduced DNA adducts in the human genome

M Yasui, Y Kanemaru, N Kamoshita, T Suzuki… - DNA repair, 2014 - Elsevier
We developed a system for tracing DNA adducts in targeted mutagenesis (TATAM) and
investigated the prevalence and types of consequent mutations. Targeted mutagenesis
methods site-specifically replace endogenous DNA bases with bases carrying synthetic
adducts using targeting vectors. The TATAM system was enabled by introduction of site-
specific DNA double strand breaks (DSB), which strongly enhanced targeting efficiency
through homologous recombination (HR), and a new polymerase chain reaction-based …