[HTML][HTML] Protein kinase Cδ-mediated phosphorylation of Connexin43 gap junction channels causes movement within gap junctions followed by vesicle internalization …
AC Cone, G Cavin, C Ambrosi, H Hakozaki… - Journal of Biological …, 2014 - ASBMB
Phosphorylation of gap junction proteins, connexins, plays a role in global signaling events
involving kinases. Connexin43 (Cx43), a ubiquitous and important connexin, has several
phosphorylation sites for specific kinases. We appended an imaging reporter tag for the
activity of the δ isoform of protein kinase C (PKCδ) to the carboxyl terminus of Cx43. The
FRET signal of this reporter is inversely related to the phosphorylation of serine 368 of Cx43.
By activating PKC with the phorbol ester phorbol 12, 13-dibutyrate (PDBu) or a natural …
involving kinases. Connexin43 (Cx43), a ubiquitous and important connexin, has several
phosphorylation sites for specific kinases. We appended an imaging reporter tag for the
activity of the δ isoform of protein kinase C (PKCδ) to the carboxyl terminus of Cx43. The
FRET signal of this reporter is inversely related to the phosphorylation of serine 368 of Cx43.
By activating PKC with the phorbol ester phorbol 12, 13-dibutyrate (PDBu) or a natural …