Advantages of single-nucleus over single-cell RNA sequencing of adult kidney: rare cell types and novel cell states revealed in fibrosis

H Wu, Y Kirita, EL Donnelly… - Journal of the American …, 2019 - journals.lww.com
Background A challenge for single-cell genomic studies in kidney and other solid tissues is
generating a high-quality single-cell suspension that contains rare or difficult-to-dissociate
cell types and is free of both RNA degradation and artifactual transcriptional stress
responses. Methods We compared single-cell RNA sequencing (scRNA-seq) using the
DropSeq platform with single-nucleus RNA sequencing (snRNA-seq) using sNuc-DropSeq,
DroNc-seq, and 10X Chromium platforms on adult mouse kidney. We validated snRNA-seq …