Complementary roles for single-nucleus and single-cell RNA sequencing in kidney disease research
ED O'sullivan, KJ Mylonas, J Hughes… - Journal of the …, 2019 - journals.lww.com
We congratulate Wu et al. 1 on their successful application of single-nucleus RNA
sequencing to characterize renal cell types underrepresented by single-cell RNA
sequencing. A particular strength of their workflow is the detection of cells such as
podocytes, mesangial cells, and glomerular endothelial cells, which can be
underrepresented by conventional single-cell sequencing. These cell types have previously
been described as distinct clusters using single-cell sequencing at markedly higher total cell …
sequencing to characterize renal cell types underrepresented by single-cell RNA
sequencing. A particular strength of their workflow is the detection of cells such as
podocytes, mesangial cells, and glomerular endothelial cells, which can be
underrepresented by conventional single-cell sequencing. These cell types have previously
been described as distinct clusters using single-cell sequencing at markedly higher total cell …