Fluorimetric assay of D-lactate
AC McLellan, SA Phillips, PJ Thornalley - Analytical biochemistry, 1992 - Elsevier
AC McLellan, SA Phillips, PJ Thornalley
Analytical biochemistry, 1992•ElsevierA fluorimetric assay for n-lactate in human blood samples was developed using an endpoint
enzymatic assay with d-lactate dehydrogenase from Staphylococcus epidermidis. The
intrabatch and interbatch coefficients of variance were 8.7%(n= 4) and 16.6%(n= 4),
respectively. The limit of detection in blood was 3.73 nmol/ml. The assay suffers minor
interference from Sd-lactoylglutathione, which was also present in the blood samples. The
concentration of n-lactate in blood was (mean±SE, nmol/ml) normal healthy individuals …
enzymatic assay with d-lactate dehydrogenase from Staphylococcus epidermidis. The
intrabatch and interbatch coefficients of variance were 8.7%(n= 4) and 16.6%(n= 4),
respectively. The limit of detection in blood was 3.73 nmol/ml. The assay suffers minor
interference from Sd-lactoylglutathione, which was also present in the blood samples. The
concentration of n-lactate in blood was (mean±SE, nmol/ml) normal healthy individuals …
A fluorimetric assay for n-lactate in human blood samples was developed using an endpoint enzymatic assay with d-lactate dehydrogenase from Staphylococcus epidermidis. The intrabatch and interbatch coefficients of variance were 8.7% (n = 4) and 16.6% (n = 4), respectively. The limit of detection in blood was 3.73 nmol/ml. The assay suffers minor interference from S-d-lactoylglutathione, which was also present in the blood samples. The concentration of n-lactate in blood was (mean ± SE, nmol/ml) normal healthy individuals, 11.0 ± 1.2 (n = 7); and diabetic patients, 20.0 ± 1.3 (n = 55) (a significant increase in diabetes mellitus; P < 0.01, Mann-Whitney U test).
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