RAGE-induced cytosolic ROS promote mitochondrial superoxide generation in diabetes

MT Coughlan, DR Thorburn, SA Penfold… - Journal of the …, 2009 - journals.lww.com
Damaged mitochondria generate an excess of superoxide, which may mediate tissue injury
in diabetes. We hypothesized that in diabetic nephropathy, advanced glycation end-
products (AGEs) lead to increases in cytosolic reactive oxygen species (ROS), which
facilitate the production of mitochondrial superoxide. In normoglycemic conditions, exposure
of primary renal cells to AGEs, transient overexpression of the receptor for AGEs (RAGE)
with an adenoviral vector, and infusion of AGEs to healthy rodents each induced renal …