[HTML][HTML] MicroRNA-205 regulates the calcification and osteoblastic differentiation of vascular smooth muscle cells

W Qiao, L Chen, M Zhang - Cellular physiology and biochemistry, 2014 - karger.com
W Qiao, L Chen, M Zhang
Cellular physiology and biochemistry, 2014karger.com
Objectives: We investigated the role of miR-205 in the osteogenic differentiation of vascular
smooth muscle cells (VSMCs). Methods: Osteogenic differentiation of human aortic smooth
muscle cells (HASMCs) was induced by 10 mM β-glycerophosphate (β-GP). Alizarin Red S
staining, alkaline phosphatase (ALP) activity and osteocalcin secretion were used to
determine osteogenic differentiation of HASMCs. Quantitative reverse transcriptase
polymerase chain reaction (qRT-PCR) was used to measure the expression of miR-205 in …
Objectives
We investigated the role of miR-205 in the osteogenic differentiation of vascular smooth muscle cells (VSMCs).
Methods
Osteogenic differentiation of human aortic smooth muscle cells (HASMCs) was induced by 10 mM β-glycerophosphate (β-GP). Alizarin Red S staining, alkaline phosphatase (ALP) activity and osteocalcin secretion were used to determine osteogenic differentiation of HASMCs. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expression of miR-205 in HASMCs.
Results
The expression of endogenous miR-205 was decreased in HASMCs during β-glycerophosphate-induced calcification. Overexpression of miR-205 inhibited the differentiation of HASMCs into osteoblast-like cells, as evidenced by a decrease in ALP activity, osteocalcin secretion, and Runx2 expression, whereas miR-205 depletion enhanced osteoblastic differentiation of HASMCs. Runx2 and Smad1 were identified as direct targets of miR-205 by computational analysis and experimental assays.
Conclusion
The present study shows that miR-205 may negatively regulate the β-glycerophosphate-induced calcification of HASMCs, at least partially by targeting Runx2 and Smad1.
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