Cytokine profile and supposed contribution to scarring in cicatricial pemphigoid
M Caproni, A Calzolari, E Salvatore… - Journal of oral …, 2003 - Wiley Online Library
M Caproni, A Calzolari, E Salvatore, B Giomi, W Volpi, A D'Agata, M Santucci, P Fabbri
Journal of oral pathology & medicine, 2003•Wiley Online LibraryBackground: The progressive scarring observed in cicatricial pemphigoid (CP) is still
partially unexplained but recently the release of soluble fibrogenic factors by inflammatory
infiltrating cells has been considered as pathogenically relevant. In the present study we
evaluated the expression of mRNA for IL‐4, IL‐5, TGF‐β1, IFN‐γ in CP in comparison to
bullous pemphigoid (BP) patients, investigating the role of cytokine profile as possible cause
of the different disease evolution. Methods: Biopsies from patients with oral (n= 10), preputial …
partially unexplained but recently the release of soluble fibrogenic factors by inflammatory
infiltrating cells has been considered as pathogenically relevant. In the present study we
evaluated the expression of mRNA for IL‐4, IL‐5, TGF‐β1, IFN‐γ in CP in comparison to
bullous pemphigoid (BP) patients, investigating the role of cytokine profile as possible cause
of the different disease evolution. Methods: Biopsies from patients with oral (n= 10), preputial …
Abstract
Background: The progressive scarring observed in cicatricial pemphigoid (CP) is still partially unexplained but recently the release of soluble fibrogenic factors by inflammatory infiltrating cells has been considered as pathogenically relevant. In the present study we evaluated the expression of mRNA for IL‐4, IL‐5, TGF‐β1, IFN‐γ in CP in comparison to bullous pemphigoid (BP) patients, investigating the role of cytokine profile as possible cause of the different disease evolution.
Methods: Biopsies from patients with oral (n = 10), preputial (n = 3) and cutaneous (n = 1) CP were studied by in situ hybridisation performing a new amplification system based on biotinyl‐tyramide. As control, four patients affected by BP were also examined, together with healthy tissue from two CP and two BP patients, respectively.
Results: In CP IL‐4 mRNA expression was present in 4 out of 14 cases analysed. IL‐5 was detected in 12 CP biopsies. TGF‐β1 and IFN‐γ mRNAs were identified in 9 and 11 CP cases, respectively. In BP, IL‐4 hybridisation signal could not be observed in any of the cases. By contrast IL‐5, TGF‐β1 and IFN‐γ mRNA analyses were positive in all BP cases. Healthy specimens did not show any expression for IL‐4, IL‐5 and IFN‐γ, while a poor staining for TGF‐β was found in epithelium and subjunctional areas.
Conclusions: Our results highlight the presence of a mixed cytokine pattern in the cellular infiltrate of both blistering diseases, with a corresponding increase of Th2‐like activity in fully developed lesions, irrespective of the different sites involved. In addition, the constant presence of TGF‐β1 mRNA in the different lesional phases of CP, and its overlapping expression in BP suggest that the involvement of additional factors is responsible for the scarring course typical of CP.
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