Employing ProteoWizard to convert raw mass spectrometry data

JD Holman, DL Tabb, P Mallick - Current protocols in …, 2014 - Wiley Online Library
JD Holman, DL Tabb, P Mallick
Current protocols in bioinformatics, 2014Wiley Online Library
After raw data have been captured by mass spectrometers in biological LC‐MS/MS
experiments, they must be converted from vendor‐specific binary files to open‐format files
for manipulation by most software. This protocol details the use of ProteoWizard software for
this conversion, taking format features, coding options, and vendor particularities into
account. This protocol will aid researchers in preparing their data for analysis by database
search engines and other bioinformatics tools. Curr. Protoc. Bioinform. 46: 13.24. 1‐13.24 …
Abstract
After raw data have been captured by mass spectrometers in biological LC‐MS/MS experiments, they must be converted from vendor‐specific binary files to open‐format files for manipulation by most software. This protocol details the use of ProteoWizard software for this conversion, taking format features, coding options, and vendor particularities into account. This protocol will aid researchers in preparing their data for analysis by database search engines and other bioinformatics tools. Curr. Protoc. Bioinform. 46:13.24.1‐13.24.9. © 2014 by John Wiley & Sons, Inc.
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