Rapid onset of intestinal epithelial and lymphocyte apoptotic cell death in patients with trauma and shock
RS Hotchkiss, RE Schmieg Jr, PE Swanson… - Critical care …, 2000 - journals.lww.com
RS Hotchkiss, RE Schmieg Jr, PE Swanson, BD Freeman, KW Tinsley, JP Cobb, IE Karl…
Critical care medicine, 2000•journals.lww.comObjective Apoptosis is a cellular suicide program that can be activated by cell injury or
stress. Although a number of laboratory studies have shown that ischemia/reperfusion injury
can induce apoptosis, few clinical studies have been performed. The purpose of this study
was to determine whether apoptosis is a major mechanism of cell death in intestinal
epithelial cells and lymphocytes in patients who sustained trauma, shock, and
ischemia/reperfusion injury. Design Intestinal tissues were obtained intraoperatively from 10 …
stress. Although a number of laboratory studies have shown that ischemia/reperfusion injury
can induce apoptosis, few clinical studies have been performed. The purpose of this study
was to determine whether apoptosis is a major mechanism of cell death in intestinal
epithelial cells and lymphocytes in patients who sustained trauma, shock, and
ischemia/reperfusion injury. Design Intestinal tissues were obtained intraoperatively from 10 …
Abstract
Objective
Apoptosis is a cellular suicide program that can be activated by cell injury or stress. Although a number of laboratory studies have shown that ischemia/reperfusion injury can induce apoptosis, few clinical studies have been performed. The purpose of this study was to determine whether apoptosis is a major mechanism of cell death in intestinal epithelial cells and lymphocytes in patients who sustained trauma, shock, and ischemia/reperfusion injury.
Design
Intestinal tissues were obtained intraoperatively from 10 patients with acute traumatic injuries as a result of motor vehicle collisions or gun shot wounds. A control population consisted of six patients who underwent elective bowel resections. Apoptosis was evaluated by conventional light microscopy, laser scanning confocal microscopy using the nuclear staining dye Hoechst 33342, immunohistochemical staining for active caspase-3, and immunohistochemical staining for cytokeratin 18.
Setting
Lippincott Williams & Wilkins