Apoptotic cell death in patients with sepsis, shock, and multiple organ dysfunction
RS Hotchkiss, PE Swanson, BD Freeman… - Critical care …, 1999 - journals.lww.com
RS Hotchkiss, PE Swanson, BD Freeman, KW Tinsley, JP Cobb, GM Matuschak…
Critical care medicine, 1999•journals.lww.comObjectives The purpose of this study was to determine whether apoptosis is a major
mechanism of cell death in patients with sepsis. The activities of caspase-3 and the
antiapoptotic protein, BCL-2, were investigated also. Design A prospective study of 20
patients who died of sepsis and multiple organ dysfunction was performed. The control
group of 16 patients consisted of critically ill, nonseptic patients who were evaluated either
prospectively [7] or retrospectively [9]. In addition, normal colon sections from seven patients …
mechanism of cell death in patients with sepsis. The activities of caspase-3 and the
antiapoptotic protein, BCL-2, were investigated also. Design A prospective study of 20
patients who died of sepsis and multiple organ dysfunction was performed. The control
group of 16 patients consisted of critically ill, nonseptic patients who were evaluated either
prospectively [7] or retrospectively [9]. In addition, normal colon sections from seven patients …
Abstract
Objectives
The purpose of this study was to determine whether apoptosis is a major mechanism of cell death in patients with sepsis. The activities of caspase-3 and the antiapoptotic protein, BCL-2, were investigated also.
Design
A prospective study of 20 patients who died of sepsis and multiple organ dysfunction was performed. The control group of 16 patients consisted of critically ill, nonseptic patients who were evaluated either prospectively [7] or retrospectively [9]. In addition, normal colon sections from seven patients who had bowel resections were included. Apoptosis was evaluated in hematoxylin and eosin-stained specimens by deoxyuridine triphosphate nick end-labeling (TUNEL) and by DNA gel electrophoresis.
Setting
Lippincott Williams & Wilkins