[PDF][PDF] Oncogenic Kras-induced GM-CSF production promotes the development of pancreatic neoplasia

Y Pylayeva-Gupta, KE Lee, CH Hajdu, G Miller… - Cancer cell, 2012 - cell.com
Y Pylayeva-Gupta, KE Lee, CH Hajdu, G Miller, D Bar-Sagi
Cancer cell, 2012cell.com
Stromal responses elicited by early stage neoplastic lesions can promote tumor growth.
However, the molecular mechanisms that underlie the early recruitment of stromal cells to
sites of neoplasia remain poorly understood. Here, we demonstrate an oncogenic Kras
G12D-dependent upregulation of GM-CSF in mouse pancreatic ductal epithelial cells
(PDECs). An enhanced GM-CSF production is also observed in human PanIN lesions. Kras
G12D-dependent production of GM-CSF in vivo is required for the recruitment of Gr1+ …
Summary
Stromal responses elicited by early stage neoplastic lesions can promote tumor growth. However, the molecular mechanisms that underlie the early recruitment of stromal cells to sites of neoplasia remain poorly understood. Here, we demonstrate an oncogenic KrasG12D-dependent upregulation of GM-CSF in mouse pancreatic ductal epithelial cells (PDECs). An enhanced GM-CSF production is also observed in human PanIN lesions. KrasG12D-dependent production of GM-CSF in vivo is required for the recruitment of Gr1+CD11b+ myeloid cells. The suppression of GM-CSF production inhibits the in vivo growth of KrasG12D-PDECs, and, consistent with the role of GM-CSF in Gr1+CD11b+ mobilization, this effect is mediated by CD8+ T cells. These results identify a pathway that links oncogenic activation to the evasion of antitumor immunity.
cell.com