Isolation and characterization of human alveolar type II cells
B Kosmider, RJ Mason, K Bahmed - Lung Innate Immunity and …, 2018 - Springer
B Kosmider, RJ Mason, K Bahmed
Lung Innate Immunity and Inflammation: Methods and Protocols, 2018•SpringerAlveolar type II (ATII) cells synthesize, store, and secrete pulmonary surfactant and restore
the epithelium after damage to the alveolar epithelium. Isolation of human ATII cells provides
a valuable tool to study their function under normal and pathophysiological conditions.
Moreover, maintenance of their differentiated phenotype in vitro allows further study of their
function. Here we describe a protocol for efficient ATII cell isolation, characterization, and
culture.
the epithelium after damage to the alveolar epithelium. Isolation of human ATII cells provides
a valuable tool to study their function under normal and pathophysiological conditions.
Moreover, maintenance of their differentiated phenotype in vitro allows further study of their
function. Here we describe a protocol for efficient ATII cell isolation, characterization, and
culture.
Abstract
Alveolar type II (ATII) cells synthesize, store, and secrete pulmonary surfactant and restore the epithelium after damage to the alveolar epithelium. Isolation of human ATII cells provides a valuable tool to study their function under normal and pathophysiological conditions. Moreover, maintenance of their differentiated phenotype in vitro allows further study of their function. Here we describe a protocol for efficient ATII cell isolation, characterization, and culture.
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