[HTML][HTML] Secreted and transmembrane mucins inhibit gene transfer with AAV4 more efficiently than AAV5
RW Walters, JM Pilewski, JA Chiorini… - Journal of Biological …, 2002 - ASBMB
Adeno-associated virus (AAV) is a promising vector for gene transfer in cystic fibrosis. AAV4
and AAV5 both bind to the apical surface of differentiated human airway epithelia, but only
AAV5 infects. Both AAV4 and AAV5 require 2, 3-linked sialic acid for binding. However,
AAV5 interacts with sialic acid onN-linked carbohydrates, whereas AAV4 interacts with sialic
acid on O-linked carbohydrates. Because mucin is decorated with O-linked carbohydrates,
we hypothesized that mucin binds AAV4 and inhibits gene transfer. To evaluate the effect of …
and AAV5 both bind to the apical surface of differentiated human airway epithelia, but only
AAV5 infects. Both AAV4 and AAV5 require 2, 3-linked sialic acid for binding. However,
AAV5 interacts with sialic acid onN-linked carbohydrates, whereas AAV4 interacts with sialic
acid on O-linked carbohydrates. Because mucin is decorated with O-linked carbohydrates,
we hypothesized that mucin binds AAV4 and inhibits gene transfer. To evaluate the effect of …