Efficient assessment of developmental, surgical and pathological lymphangiogenesis using a lymphatic reporter mouse and its embryonic stem cells

M Hong, E Jung, S Yang, W Jung, YJ Seong, E Park… - PloS one, 2016 - journals.plos.org
M Hong, E Jung, S Yang, W Jung, YJ Seong, E Park, A Bramos, KE Kim, S Lee, G Daghlian
PloS one, 2016journals.plos.org
Several lymphatic reporter mouse lines have recently been developed to significantly
improve imaging of lymphatic vessels. Nonetheless, the usage of direct visualization of
lymphatic vessels has not been fully explored and documented. Here, we characterized a
new Prox1-tdTomato transgenic lymphatic reporter mouse line, and demonstrated how this
animal tool enables the researchers to efficiently assess developmental, surgical and
pathological lymphangiogenesis by direct visualization of lymphatic vessels. Moreover, we …
Several lymphatic reporter mouse lines have recently been developed to significantly improve imaging of lymphatic vessels. Nonetheless, the usage of direct visualization of lymphatic vessels has not been fully explored and documented. Here, we characterized a new Prox1-tdTomato transgenic lymphatic reporter mouse line, and demonstrated how this animal tool enables the researchers to efficiently assess developmental, surgical and pathological lymphangiogenesis by direct visualization of lymphatic vessels. Moreover, we have derived embryonic stem cells from this reporter line, and successfully differentiated them into lymphatic vessels in vivo. In conclusion, these experimental tools and techniques will help advance lymphatic research.
PLOS