Reversibility of the mitochondrial isocitrate dehydrogenase reaction in the perfused rat liver. Evidence from isotopomer analysis of citric acid cycle intermediates.
C Des Rosiers, CA Fernandez, F David… - Journal of Biological …, 1994 - ASBMB
The reversal of the mitochondrial isocitrate dehydrogenase reaction was investigated in rat
livers perfused with [U-13C5] glutamate or [U-13C5] glutamine. The mass isotopomer
distribution of citric acid cycle intermediates extracted from the livers was determined by gas
chromatography-mass spectrometry. Citrate was enriched in an isotopomer containing five
13C. The formation of this isotopomer can only be explained by the reversal of the isocitrate
dehydrogenase reaction. Calculation of kinetic parameters from the mass isotopomer data …
livers perfused with [U-13C5] glutamate or [U-13C5] glutamine. The mass isotopomer
distribution of citric acid cycle intermediates extracted from the livers was determined by gas
chromatography-mass spectrometry. Citrate was enriched in an isotopomer containing five
13C. The formation of this isotopomer can only be explained by the reversal of the isocitrate
dehydrogenase reaction. Calculation of kinetic parameters from the mass isotopomer data …