[HTML][HTML] Impaired tricarboxylic acid cycle activity in mouse livers lacking cytosolic phosphoenolpyruvate carboxykinase
SC Burgess, N Hausler, M Merritt, FMH Jeffrey… - Journal of Biological …, 2004 - ASBMB
Liver-specific phosphoenolpyruvate carboxykinase (PEPCK) null mice, when fasted,
maintain normal whole body glucose kinetics but develop dramatic hepatic steatosis. To
identify the abnormalities of hepatic energy generation that lead to steatosis during fasting,
we studied metabolic fluxes in livers lacking hepatic cytosolic PEPCK by NMR using 2 H and
13 C tracers. After a 4-h fast, glucose production from glycogenolysis and conversion of
glycerol to glucose remains normal, whereas gluconeogenesis from tricarboxylic acid (TCA) …
maintain normal whole body glucose kinetics but develop dramatic hepatic steatosis. To
identify the abnormalities of hepatic energy generation that lead to steatosis during fasting,
we studied metabolic fluxes in livers lacking hepatic cytosolic PEPCK by NMR using 2 H and
13 C tracers. After a 4-h fast, glucose production from glycogenolysis and conversion of
glycerol to glucose remains normal, whereas gluconeogenesis from tricarboxylic acid (TCA) …