[HTML][HTML] Imaging mass spectrometry demonstrates age-related decline in human adipose plasticity

C Guillermier, PK Fazeli, S Kim, M Lun, JP Zuflacht… - JCI insight, 2017 - ncbi.nlm.nih.gov
C Guillermier, PK Fazeli, S Kim, M Lun, JP Zuflacht, J Milian, H Lee, H Francois-Saint-Cyr…
JCI insight, 2017ncbi.nlm.nih.gov
Quantification of stable isotope tracers has revealed the dynamic state of living tissues. A
new form of imaging mass spectrometry quantifies isotope ratios in domains much smaller
than a cubic micron, enabling measurement of cell turnover and metabolism with stable
isotope tracers at the single-cell level with a methodology we refer to as multi-isotope
imaging mass spectrometry. In a first-in-human study, we utilize stable isotope tracers of
DNA synthesis and de novo lipogenesis to prospectively measure cell birth and adipocyte …
Abstract
Quantification of stable isotope tracers has revealed the dynamic state of living tissues. A new form of imaging mass spectrometry quantifies isotope ratios in domains much smaller than a cubic micron, enabling measurement of cell turnover and metabolism with stable isotope tracers at the single-cell level with a methodology we refer to as multi-isotope imaging mass spectrometry. In a first-in-human study, we utilize stable isotope tracers of DNA synthesis and de novo lipogenesis to prospectively measure cell birth and adipocyte lipid turnover. In a study of healthy adults, we elucidate an age-dependent decline in new adipocyte generation and adipocyte lipid turnover. A linear regression model suggests that the aging effect could be mediated by a decline in insulin-like growth factor-1 (IGF-1). This study therefore establishes a method for measurement of cell turnover and metabolism in humans with subcellular resolution while implicating the growth hormone/IGF-1 axis in adipose tissue aging.
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