CD61 enriches long-term repopulating hematopoietic stem cells
T Umemoto, M Yamato, Y Shiratsuchi… - Biochemical and …, 2008 - Elsevier
T Umemoto, M Yamato, Y Shiratsuchi, M Terasawa, J Yang, K Nishida, Y Kobayashi…
Biochemical and biophysical research communications, 2008•ElsevierAmong the subsets that define hematopoietic stem cells (HSCs), CD34− c-kit+ Sca-1+
lineage marker−(CD34− KSL) cells are regarded as one of the populations that have the
highest enrichment of HSCs in adult mouse bone marrow. Here, we demonstrate that long-
term repopulating hematopoietic stem cells (LTR-HSCs) have high expression of CD61
(integrin β3) within the CD34− KSL population. Approximately 60% of CD34− KSL cells
showed high expression of CD61. CD61HighCD34− KSL populations also exhibited …
lineage marker−(CD34− KSL) cells are regarded as one of the populations that have the
highest enrichment of HSCs in adult mouse bone marrow. Here, we demonstrate that long-
term repopulating hematopoietic stem cells (LTR-HSCs) have high expression of CD61
(integrin β3) within the CD34− KSL population. Approximately 60% of CD34− KSL cells
showed high expression of CD61. CD61HighCD34− KSL populations also exhibited …
Among the subsets that define hematopoietic stem cells (HSCs), CD34− c-kit+ Sca-1+ lineage marker− (CD34−KSL) cells are regarded as one of the populations that have the highest enrichment of HSCs in adult mouse bone marrow. Here, we demonstrate that long-term repopulating hematopoietic stem cells (LTR-HSCs) have high expression of CD61 (integrin β3) within the CD34−KSL population. Approximately 60% of CD34−KSL cells showed high expression of CD61. CD61HighCD34−KSL populations also exhibited significantly greater properties of HSC, such as expression of HSC markers, the side population (SP) phenotype, and ability for long-term repopulation. In both SP cells and non-SP (NSP) cells, CD61HighCD34−KSL cells also contained significantly more LTR-HSCs than CD61Low/−CD34−KSL cells. Our results indicate that CD61 is exploitable for HSC enrichment as a supportive positive cell surface marker.
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