Detection of apoptosis by TUNEL assay

K Kyrylkova, S Kyryachenko, M Leid… - … : methods and protocols, 2012 - Springer
K Kyrylkova, S Kyryachenko, M Leid, C Kioussi
Odontogenesis: methods and protocols, 2012Springer
Terminal deoxynucleotidyl transferase (T dT) d U TP N ick-E nd L abeling (TUNEL) assay
has been designed to detect apoptotic cells that undergo extensive DNA degradation during
the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of
double-stranded DNA breaks independent of a template. This chapter describes an assay
for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL
system.
Abstract
Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.
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