Barcoded cDNA library preparation for small RNA profiling by next-generation sequencing

M Hafner, N Renwick, TA Farazi, A Mihailović… - Methods, 2012 - Elsevier
M Hafner, N Renwick, TA Farazi, A Mihailović, JTG Pena, T Tuschl
Methods, 2012Elsevier
The characterization of post-transcriptional gene regulation by small regulatory (20–30 nt)
RNAs, particularly miRNAs and piRNAs, has become a major focus of research in recent
years. A prerequisite for characterizing small RNAs is their identification and quantification
across different developmental stages, and in normal and disease tissues, as well as model
cell lines. Here we present a step-by-step protocol for generating barcoded small RNA
cDNA libraries compatible with Illumina HiSeq sequencing, thereby facilitating miRNA and …
The characterization of post-transcriptional gene regulation by small regulatory (20–30 nt) RNAs, particularly miRNAs and piRNAs, has become a major focus of research in recent years. A prerequisite for characterizing small RNAs is their identification and quantification across different developmental stages, and in normal and disease tissues, as well as model cell lines. Here we present a step-by-step protocol for generating barcoded small RNA cDNA libraries compatible with Illumina HiSeq sequencing, thereby facilitating miRNA and other small RNA profiling of large sample collections.
Elsevier