Heparin and EDTA as anticoagulant differentially affect cytokine mRNA level of cultured porcine blood cells

JC Duvigneau, W Sipos, RT Hartl, M Bayer… - Journal of …, 2007 - Elsevier
Cytokine mRNA expression profiles serve to characterize immune cell activation in different
test systems. Both, diluted whole blood and isolated PBMC are widely applied for these
studies. Comprehensive data regarding the suitability of different anticoagulants for profiling
cytokine expression are not available for the pig. Therefore the aim of this study was to
compare the effect of two commonly used anticoagulants (heparin and EDTA) on the
cytokine expression pattern of porcine blood cells. IL-1α, IL-2, IL-4, IL-6, IL-10 and IFN-γ …