Bioinformatic analysis of barcoded cDNA libraries for small RNA profiling by next-generation sequencing
TA Farazi, M Brown, P Morozov, JJ Ten Hoeve… - Methods, 2012 - Elsevier
Methods, 2012•Elsevier
The characterization of post-transcriptional gene regulation by small regulatory RNAs of 20–
30 nt length, particularly miRNAs and piRNAs, has become a major focus of research in
recent years. A prerequisite for the characterization of small RNAs is their identification and
quantification across different developmental stages, normal and diseased tissues, as well
as model cell lines. Here we present a step-by-step protocol for the bioinformatic analysis of
barcoded cDNA libraries for small RNA profiling generated by Illumina sequencing, thereby …
30 nt length, particularly miRNAs and piRNAs, has become a major focus of research in
recent years. A prerequisite for the characterization of small RNAs is their identification and
quantification across different developmental stages, normal and diseased tissues, as well
as model cell lines. Here we present a step-by-step protocol for the bioinformatic analysis of
barcoded cDNA libraries for small RNA profiling generated by Illumina sequencing, thereby …
The characterization of post-transcriptional gene regulation by small regulatory RNAs of 20–30 nt length, particularly miRNAs and piRNAs, has become a major focus of research in recent years. A prerequisite for the characterization of small RNAs is their identification and quantification across different developmental stages, normal and diseased tissues, as well as model cell lines. Here we present a step-by-step protocol for the bioinformatic analysis of barcoded cDNA libraries for small RNA profiling generated by Illumina sequencing, thereby facilitating miRNA and other small RNA profiling of large sample collections.
Elsevier
