Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins

K Tashiro, H Tada, R Heilker, M Shirozu, T Nakano… - Science, 1993 - science.org
K Tashiro, H Tada, R Heilker, M Shirozu, T Nakano, T Honjo
Science, 1993science.org
A method was developed to clone, without the use of specific functional assays,
complementary DNAs (cDNAs) that carry specific amino-terminal signal sequences, such as
those encoding intercellular signal-transducing molecules and receptors. The vector used in
this system directed the cell surface expression of interleukin-2 receptor fusion proteins
when inserts with signal sequences were cloned in-frame with the correct orientation. An
expression cDNA library was constructed from a bone marrow stromal cell line, which …
A method was developed to clone, without the use of specific functional assays, complementary DNAs (cDNAs) that carry specific amino-terminal signal sequences, such as those encoding intercellular signal-transducing molecules and receptors. The vector used in this system directed the cell surface expression of interleukin-2 receptor fusion proteins when inserts with signal sequences were cloned in-frame with the correct orientation. An expression cDNA library was constructed from a bone marrow stromal cell line, which contained 5′ portion-enriched cDNAs (the average size was 400 base pairs). Two cDNAs that encoded putative cytokine molecules, stromal cell-derived factor-1α (SDF-1α) and SDF-1β, which belong to the intercrine-macrophage inflammatory protein superfamily, were cloned.
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